The IQFISH panels include a set of oligonucleotide-based ready-to-use FISH probes, premixed with IQFISH hybridization buffer, for the detection of gene rearrangements and for the detection of gene amplifications by fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The probes consist of two child probes (3 and 5 probes), each designed to target a given gene and each labeled in a different color (orange-red Cy3 and green FITC).
The assays combine two ground-breaking technologies: oligonucleotide-based SureFISH technology and the formamide-free IQFISH hybridization buffer. SureFISH technology enables chromosomal aberration detection using a precise, synthetic oligonucleotide probe design process. IQFISH hybridization buffer is a unique, ultra-fast hybridization technology that allows probe hybridization to be performed in just 1-2 hours and provides complete FISH results in about 4 hours. Together these advancements provide fast, high-quality FISH results.